06 Oct 2017 19:45
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Nucleic acids are bio-molecules which might be found in cells of all residing organisms, be it plants or animals. Nucleic acids has a key role in the continuation of life in any residing organism. Nucleic acids include DNA and RNA as their major components. DNA and RNA are essential components seen in the cells of the living microorganisms that plays a major function in manufacture of necessary protein through a process of replication i. e. DNA replication.
Nucleic acids can be extracted by means of different methods, some of the methods were demonstrated by the well known Swiss physician, Friedrick Miescher in 1869 when he had been determined to solve the issue of how does the basic basic principle of life works? Since nucleic acids are compounds with unique characteristics, can make them to be able to be known from other molecules hence able to be extracted. Before these nucleic acids are being extracted, some other bio-molecules such as bacteriophages i term as " pollutants, " should be removed for you to facilitate faster extraction associated with pure nucleic acids. DNA can be extracted using recombinant method. This method involves becoming a member of together two different types of GENETIC MATERIAL molecules from different variety. This method ensures faster removal of specific DNA molecule within the organism since the two types connected with DNA materials are international to each other hence extraction involving purified DNA molecule.
Several manual methods which includes centrifugal method can be used to ensure suitable extraction of DNA as well as RNA bio-molecules, this method involves repeated centrifuge of healthy proteins materials such as blood along with materials such as egg yolks, this is done in repeated occasions until the impurities in the healthy proteins materials are removed then the pure nucleic molecule is usually remained with. However always purification of these proteins is significant in this method to ensure creation of the best product.
Solution primarily based method is a modern method that is certainly popularly being adopted by several modern physician around the globe, this method is a method that was developed by a series of famous health professionals from older generation to date. this technique involves removal of unwanted components in the solution by usage of certain specific and more stylish solutions. these solutions tend to be added to break the attachment between and among the nucleic acid. This according to their own method of bonding will result in the extraction of more purified nucleic acids than the preceding methods as stated above.
In addition an extraction kit that contains a bead that utilizes the principle of a magnet can be used to extract nucleic gastric acids from different types of the creatures this method is more efficient plus the cheapest method of all the other approaches. The magnetic rod just attract to certain certain DNA and RNA bio-molecules hence production of a pure nucleic molecules.
Other methods such as anion exchange supplies, Ion exchange chromatography and also affinity are used to extract nucleic acids, these methods are mostly laboratory methods which utilizes certain principles and a furthermore follow strict procedure. there is an automated method of extraction which will utilizes a very expensive musical instrument that can easily extract nucleic acids.
automated nucleic acid extraction